Need 11 medical technologist in Gajipur..
canndidate have to complete 3/4 years diploma from any govt supported medical technology institute..
সোমবার, ৭ ডিসেম্বর, ২০১৫
Need 11 Medical Technologist( Lab,Radiotherapy,Physiotherapy) in Shahid Tajuddin Ahmed Medical College Hospital, Gajipur.
Serum Lipid Profile Test
Total Cholesterol (TC) Directly linked to risk of heart and blood vessel disease. Goal values:75-169 mg/dL for those age 20 and younger100-199 mg/dL for those over age 21Preparation:This test may be measured any time of the day without fasting. However, if the test is drawn as part of a total lipid profile, it requires a 12-hour fast (no food or drink, except water). For the most accurate results, wait at least two months after a heart attack, surgery, infection, injury or pregnancy to check cholesterol levels. Cholesterol is a type of fat, found in your blood. It is produced by your body and also comes from the foods you eat (animal products). Cholesterol is needed by your body to maintain the health of your cells. Too much cholesterol leads to coronary artery disease. Your blood cholesterol level is related to the foods you eat or to genetic conditions (passed down from other generations of family members).High Density Lipoprotein (HDL) “Good cholesterol”High levels linked to a reduced risk of heart and blood vessel disease. The higher your HDL level, the better.Goal value:Greater than 40 mg/dL Preparation:This test may be measured any time of the day without fasting. However, if the test is drawn as part of a total lipid profile, it requires a 12-hour fast (no food or drink, except water). For the most accurate results, wait at least two months after a heart attack, surgery, infection, injury or pregnancy to check HDL levels. HDL is a lipoprotein (a combination of fat and protein) found in the blood. It is called "good" cholesterol because it removes excess cholesterol from the blood and takes it to the liver. A highHDL level is related to lower risk of heart and blood vessel disease. Low Density Lipoprotein (LDL) “Bad cholesterol”High levels are linked to an increased risk of heart and blood vessel disease, including coronary artery disease, heart attack and death. Reducing LDL levels is a major treatment target for cholesterol-lowering medications. Goal values:Less than 70 mg/dL for those with heart or blood vessel disease and for other patients at very high risk of heart disease (those with metabolic syndrome)Less than 100 mg/dL for high risk patients (e.g., some patients who have multiple heart disease risk factors)Less than 130 mg/dL for individuals who are at low risk for coronary artery disease Preparation:Blood should be collected after a 12-hour fast (no food or drink, except water). For the most accurate results, wait at least two months after a heart attack, surgery, infection, injury or pregnancy to check LDL levels. LDL is a lipoprotein (a combination of fat and protein) found in the blood. It is called "bad" cholesterol because it picks up cholesterol from the blood and takes it to the cells. A high LDL level is related to a higher risk of heart and blood vessel disease. Triglycerides (TG)Elevated in obese or diabetic patients. Level increases from eating simple sugars or drinking alcohol. Associated with heart and blood vessel disease. Goal value:Less than 150 mg/dl Preparation:Blood should be collected after a 12-hour fast (no food or drink, except water). For the most accurate results, wait at least two months after a heart attack, surgery, infection, injury or pregnancy to check triglyceride levels. Triglycerides are a type of fat found in the blood. The blood level of this type of fat is most affected by the foods you eat (such as sugar, fat or alcohol) but can also be high due to being overweight, having thyroid or liver disease and genetic conditions. High levels of triglycerides are related to a higher risk of heart and blood vessel disease.
Genetic Test Helps Some Women with Breast Cancer Avoid Chemotherapy
A 21-genetest is accurate in predicting which women diagnosedwith certain types of breast cancer will do well with hormone therapy alone and can forego chemotherapy, according to a recent study in theNew England Journal of Medicine. The ongoing study, called the Trial Assigning IndividuaLized Options for Treatment (Rx), or TAILORx, provides new evidence on the test called Oncotype DX.The test, already on the market in the United States and other countries, examines the activity of 21 genes in tumor samples taken from women during breast cancer surgery. The results are used to calculate a score between 0 and 100. The lower the score, the lower the chance the cancer will spread if only hormone therapy is prescribed, such as the drug tamoxifen.The clinical trial was conducted at multiple medical centers and enrolled over 10,000 women. The women all had a type of breast cancer that would likely respond to hormone therapy (ER/ PR positive, HER2/neu negatives) and their cancer had not spread to the lymph nodes. (Cancer spread to lymph nodes is usually a reason to have chemotherapy.) However, the women's tumors had other features (large size, intermediate or high grade) that indicated that chemotherapy might be needed in addition to hormone therapy.Women in the trial whose tumors scored 10 or lower on the Oncotype DX test received standard hormone therapy but did not undergo chemotherapy. Almost 16% of the women in the study had a score of 10 or lower. They were monitored closely for five years. At that point, the researchers determined that overall survival rate was 98% for this group and the risk that the cancer would return was 6% at five years."This should provide a lot of reassurance to women and their physicians," says the study's senior author, Kathy S. Albain M.D., Professor of Medicine at the Loyola University School of Medicine. "In women whose breast cancer scored low on the multigene test, there was outstanding survival with endocrine (hormone) therapy alone. The test provides us with greater certainty of who can safely avoid chemotherapy," said Albain.A question was raised about difference in the "low-risk" cutoff value used in the study and the standard one used in clinical practices currently. In an editorial that appeared in the same issue ofNew England Journal of Medicine, Clifford Hudis, MD, head of the Breast Cancer Service at Memorial Sloan Kettering Cancer Center wrote, "For patients in this new 'lower risk' group, it is clearly helpful, if broadly anticipated. However, for the many physicians already using the test, the gap between this cutoff point of 10 and the higher 'standard' cutoff point of 18 may be a concern."Hudis adds that while more tests, and less expensive tests, for this use are likely to be developed, "For now, however, this assay is the most rigorously tested option and provides proof of the principle that we can develop reproducible predictive tests to select patients who should not receive chemotherapy. In that regard, it is one more step toward precision, there are more steps ahead."An additional 68% of women in the study had a mid-range score of 11 to 25. This second set of women were randomly assigned to receive either hormone therapy plus chemotherapy or hormone therapy alone. These women are still being followed and results are not yet available.
"collected "
মঙ্গলবার, ৪ আগস্ট, ২০১৫
Nedd Medical Technologist At Gastroliver Hospital & Research Ltd.
Medical Technologist, Transfusion Medicine (Blood Bank)
No. of Vacancies
- 04
Job Location
- Dhaka
Salary Range
- Negotiable
Other Benefits
- As per company Policy
Please mention the title of the position in the subject line or on the top of the envelope.
Only short-listed candidates will be called for interview.
Applicant must enclose his/her Photograph with CV.
Application Deadline : Aug 20, 2015
Application Deadline : Aug 20, 2015
Company Information
Gastro Liver Hospital & Research Institute Ltd.Address : 77/A, West Panthapath, East Rajabazar, Dhaka - 1215
Web : www.brbcable.com
Business : Hospital
বুধবার, ১ জুলাই, ২০১৫
Need A Medical Lab Technologist (Male/Female)
Welcare Group
|
No. of Vacancies
- 01
Job Description / Responsibility
- Reportable to Diagnostic In charge.
- Position Location At clinic/ diagnostic level
- Capable of good pathological test.
- Prepare customers for clinical/ diagnostic services.
- Job location: Staff Quarter, Demra, Dhaka.
Job Nature
- Full-time
Educational Requirements
- Diploma In Pathology
Experience Requirements
- 1 to 2 year(s)
Additional Job Requirements
- Age 22 to 35 year(s)
- Smart, Presentable, Energetic & Hard Working.
- Computer proficiency in Ms Word, Excel & better typing speed.
Job Location
- Dhaka
Salary Range
- Negotiable
Published On:
Jun 25, 2015
No. of Vacancies :
01
Job Nature :
Full-time
Experience:
1 to 2 year(s)
Age :
22 to 35 year(s)
Job Location :
Dhaka
Salary Range:
Negotiable
Application Deadline :
Jul 25, 2015
Job Source
শনিবার, ২০ জুন, ২০১৫
ANA
ANA: Anti
nuclear antibodies (ANA) is single stand. Important for the differential
diagnosis rheumatic diseases.
Indication:
1. SLE
(systemic lupus erythematosus)
Principle: serum sample diluted 1:101 are incubated in the
microplates coated with the specific antigen. Patient’s antibodies, if present
in the specimen, bind to the antigen. The unbound fraction is washed off in the
following step. Afterwards anti-human immunoglobulins conjugated to horseradish
peroxides (conjugate) are incubated and react with the antigen-antibody complex
of the samples in the microplates.Unbound conjugate is washed off in following
step. Addition of MTB substrate generates an enzymatic colorimetric (blue)
reaction. Which is stopped by diluted acid (color changes to yellow).the rate
of color for mation from the chromogen is function of the amount of conjugate
bound to the antigen-antibody complex and this is proportional to the initial
concentration of the respective antibodies in the patient sample.
Sample dilute:
1. Serum
10 ul
2. Sample
buffer 1000 ul
Wash buffer:
1. dilute
wash buffer 20 ml
2. distiled water 980 ml
Test procedure:
1. 100
ul patients dilute serum in to the designated microwells.
2. 100
ul cut-off calibrator and negative and positive controls in to the designated
wells.
3. Incubate
for 30 minutes at 20-32 degree C
4. Wash
3 times with washing buffer.
5. 100
ul conjugate in to well.
6. Incubate
for 30 minutes at 20-32 C
7. Wash
3 times with washing buffer
8. 100
ul TMB substrate in to each well.
9. 100
ul stop solution in to each well.
10. Incubate 5
minutes
11. Read
absorbance at 450 nm within 30 minutes.
Normal range:
1. Negative : 1-12 u/ml
2. Equivocal : 12-18 u/ml
3. Positive
: >18 u/ml
সোমবার, ১৫ জুন, ২০১৫
Tanvir's Important Reagent Preparation of All Department
 |
| Reagent Preparation in Lab |
01. 20 % Sulphosalicylic acid: For Urine protein
Sulphosalicylic acid ---------20 gm
Distilled water ---------------100 ml
02. 3% Glacial acetic acid: For Urine protein
Glacial acetic
acid----------- 03 ml
Distilled
water-------------- 100 ml
03.. Barium chloride (10% W/V): For Urine bile pigment
Barium chloride ------------
10 gm
Distilled
water---------------100 ml
04. Fouchets reagent: For urine Bile pigment
Trichloroacetic
acid--------- 25 gm
10% Ferric
chloride--------- 10 ml
Distilled water
------------- 100 ml
05. Ehrlich reagent: For Urine Urobilinogen
test
4-dimethylaminobenzaldehyde--
04 gm
Concentrated
HCl-------------- 40 gm
Distilled
water----------------- 160 ml
06. 3%
Sulphosalycylic acid: For Urine Myoglobin
Sulphosalycylic
acid-------03 gm
Distilled
water------------100 ml
07. Sodium nitroprusside reagent: For Urine
ketonebodies
Ammonium sulphate-----------------100gm
Sodium carbonates anhydrous ------50 gm
Sodium nitroprusside--------------3.0 gm
Note:
Grind
the crystals to a powder and use strong glass or plastic tube.
08. 20 % Potassium chromate: For Urine Chloride
Potassium
chromate-------20 gm
Distilled
water----------- 100 ml
09. 2.9 % Silver nitrate: For Urine Chloride
Silver nitrate
-------------- 29 gm
Distilled water------------ 1000 ml
10. Benzideine reagent: For Detection of blood
(Urine, stool)
Glacial acetic acid -------1.0 ml
Benzidine powder------ knife point
Hydrogen peroxide----- 1.0 ml
Note:
First
acid mixed with benzidine powder then equal volume H202
11. 3.5% Formal Saline: For Semen dilution
Sodium bicarbonate------- 05 gm
35% Formalin------------ 01 ml
Distilled water up to ---- 100 ml
12. Selivanoffs Reagent: For Semen Fructose
Resorcinol powder --------0.5 gm
HCL------------------------- 33 ml
Distilled water up to ---- 100 ml
13.
WBC Diluting fluid: For WBC’s count
Crystal violet ------------- 0.2 gm
Glacial acetic acid -------- 40 ml
Distilled water
---------- 200 ml
14.
CSF Diluting fluid: For CSF cell count
Crystal violet ------------ 0.2 gm
Glacial acetic acid ------- 10 ml
Distilled water
---------- 90 ml
15. Pandys reagent: For CSF Globulin
Phenol
crystal-------------10 gm
Distilled
water----------- 100 ml
16. 3% Trichloroacetic acid: For CSF Protein
Trichloroacetic
acid----- 03 ml
Distilled water----------
100 ml
17. 5% Potassium chromate: For CSF Chloride
Potassium
chromate-----05 gm
Distilled
Water---------- 100 ml
18. 0.5814% Silver nitrate: For CSF Chloride
Silver
ritrate---------- 5.814 gm
Distilled
water-------- 1000 ml
19. Carbol Fuchsine stain: For Z N stain
Basic fuchsine-----------10
gm
Ethanol or methanol
absolute--100 ml
Phenol-------------------50
gm
Distilled
water----------1000 ml
Here Basic Fushsin + Alconol and Phenol+ Distelled
water. Then mixed all .
20. 20 % Sulfuric acid: For Z N Stain
Sulfuric acid -----------20
ml
Distilled water-------- 100
ml
Note:
First
Distilled Water in container then sulfuric acid mixed slowly and cool water
flow out of the container as possible.
21. Methylene blue: For Z N Stain
Ethanol
----------------- 200 ml
M blue
------------------ 12 gm
Distilled
water ---------1000 ml
Here,
200 ml + 12 gm mixed, then add 1/Ltre (1000 ML) Distilled water.
22. Crystal Violet: For Gram stain
Crystal
violet--------------20 gm
Ammonium
oxalate------09 gm
Ethanol or
Methanol, absolute ----95 ml
Distilled
water----------- 1000 ml
23. Lugols iodine: For Grams stain
Potassium
iodide---------20 gm
Iodine---------------------10
gm
Distilled water
-------- 1000 ml
Here
First Potassium iodide D/water: Then iodine mixed.
24. Iodine Solu: For Faecal preparation
Potassium
iodide-------20 gm
Iodine--------------------10
gm
Distilled water
------ 1000 ml
Here,
First potassium iodide + Distilled water Then Idine mixed Keep it brown buttle.
25. Methyl red solution:
Methyl
red--------0.5 gm
Ethanol
absolute---28 ml
Distilled water
---- 22 ml
Here
Methyl red+ Ethanol add D/Water. Keep it dark place.
26. Neissers stain (Solution-A)
For corynebacterium diptherae
(KLB)
Methylene blue ---------01 gm
Absolute alcohol-------- 50 ml
Glacial acetic acid ------ 50 ml
Distilled water --------- 1000 ml
27. Neisser’s stain (Solution – B): For KLB
Absolute alcohol --------10 ml
Distilled water --------- 300 ml
28. Neisser s solution: For Working
Solution A
-------02 Parts
Solution B
-------01 Part
Note: Mixed well and then use Filter with Filter
paper should be done before use.
29. Giemsa Stain: For Stain blood parasites
Giemsa Powder -------- 3.8 gm or
0.75 gm
Glyerine----------------- 250 ml or
35 ml
Methanol---------------- 250 ml or
65 ml
Mixed well and keep it brow bottle
Note. First Giemsa grind with glycerin and incubation
at 60 c for 02 hours.
Then
cool it and mixed Methanol
30. Crystal Violet: For capsule stain
Crystal violet----------01 gm
Distilled water -------100 ml
31. Acetone alcohol decolorize:
Acetone ----------------500 ml
Ethanol or methanol, absolute-----------475 ml
Distilled water------- --25 ml
32. Ammonium hydroxide, 30% v/v
Ammonia Solution conc.--15 ml
Distilled water--------------35 ml
33. Neutral red solution:
Neutral red--------------01 gm
Distilled water----------01 litre
34. Phenol saturated solution:
Phenol crystal---------- 02 gm
Distilled water--------- 30 ml
35. Tri sodium citrate (3.8% w/v): For ESR, PT
Tri sodium citrate --------3.8 gm
Distilled water----------- 100 ml
36. EDTA (Ethylene diamine tetra acetic acid):
For CP
Dipotassium ethylene diamine tetra acetate--20 gm
Distilled water---------------- 200 ml
37. Drabkins solution: Haemoglobin estimation.
Potassium ferricyanide ----------200 mg
Potassium cyanide -----------------50 mg
Potassium dihydrogen phosphate –140 mg
Nouidet P40 (Shell chemical co) – 01 ml
Distilled water ------------------1000 ml
Note: Keep it at room temperature in brown glass
bottle.
38. Crysodine solution: For Neister stain
Crysodine powder---1.0 gm
Distilled water ------100 ml
Note:
Dissolve by gently heating and filter and then use.
39. Leishman’s stain-
Leishman powder------0.15 gm
Acetone free methyl alcohol-- 100 ml
40. WBC Count fluid of blood-
Glacial acetic acid ---------1.5 ml
01% aqueous solution of Gention violet- 01 ml
Distilled water ------------------100 ml
41. RBC Count fluid of blood-
Sodium citrate --------------3.0 gm
Formalin---------------------- 01 ml
Distilled water --------------100 ml
42. Eosinophil fluid-
Eosin ------------------------ 0.1 gm
Acetone----------------------- 10 ml
Distilled water --------------100 ml
43. Platelet count fluid-
Ammonium oxalate---------01 gm
Distilled water --------------100 ml
44. 0.5 % Eosin solution : For Faccal preparation
Eosin powder--------------------0.5 gm
Distilled water -----------------100 ml
45. 60 Copper sulphate solution : For Specific gravity (Aspiration fluid)
Stock Solution:
Copper sulphate-----------------159 gm
Distilled water ------------------1000 ml
Working solution
|
Stock
Solution
|
Distilled
Water
|
Specific
Gravity
|
|
09ml
|
91ml
|
1010
|
|
10ml
|
90ml
|
1011
|
|
11ml
|
89ml
|
1012
|
|
12ml
|
88ml
|
1013
|
|
13ml
|
87ml
|
1014
|
|
14ml
|
86ml
|
1015
|
|
15ml
|
85ml
|
1016
|
|
16ml
|
84ml
|
1017
|
|
17ml
|
83ml
|
1018
|
|
18ml
|
82ml
|
1019
|
|
19ml
|
81ml
|
1020
|
|
20ml
|
80ml
|
1021
|
|
21ml
|
79ml
|
1022
|
|
22ml
|
78ml
|
1023
|
|
23ml
|
77ml
|
1024
|
|
24ml
|
76ml
|
1025
|
|
25ml
|
75ml
|
1026
|
|
26ml
|
74ml
|
1027
|
|
27ml
|
73ml
|
1028
|
|
28ml
|
72ml
|
1029
|
|
29ml
|
71ml
|
1030
|
FORMULA FOR
TOTAL PROTEIN
Sp
Gravity-1007 369 - 1000 = gm/dl
Ref Book :
Practical Clinical Biochemistry by Harold
Varely (4
th Ed ) Page no – 242
|
Sp
Gravity
|
Protein
(gm/dl)
|
|
1012
|
1.80
gm/dl
|
|
1013
|
2.20
gm/dl
|
|
1014
|
2.55
gm/dl
|
|
1015
|
2.90
gm/dl
|
|
1016
|
3.30
gm/dl
|
|
1017
|
3.65
gm/dl
|
|
1018
|
4.00
gm/dl
|
|
1019
|
4.40
gm/dl
|
|
1020
|
4.70
gm/dl
|
|
1021
|
5.20 gm/dl
|
|
1022
|
5.50
gm/dl
|
|
1023
|
5.80
gm/dl
|
|
1024
|
6.20
gm/dl
|
|
1025
|
6.60
gm/dl
|
|
1026
|
6.90
gm/dl
|
|
1027
|
7.30
gm/dl
|
|
1028
|
7.70
gm/dl
|
|
1029
|
8.00
gm/dl
|
|
1030
|
8.40
gm/dl
|
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