WOUND SWAB CULTURE
DAY 1
Naked eye examination:
The pus of a staphylococcal lesion is typically creamy and thick in consistency.
That of streptococcus is watery.
That of proteus has a fishy smell and that of pseudomonas infection a sweet, musty odour and pigmented.
Pus containing an-aerobic organisms often has an offensive putrid smell.
Inoculate the wound swab in-
Blood agar both aerobic and an-aerobic condition.
MacConkey’s agar.
Robertson cooked meat media
All the media incubated at 370 c for 24 hours aerobically/anaerobically.
Gram staining:
Z-N staining.
DAY 2
Inspection of the inoculating media:
To see colony morphology.
Blood agar-
Staph aureus- pin head colonies.
Pseudomonas – black colonies
MacConkey’s agar
Gram staining.
Motility:
Subculture in nutrient agar.
For biochemical tests- Sugar set and API-20E.
For drug sensitivity: Disc sensitivity test
If Gram positive cocci: Catalase, coagulase test.
DAY 3
Inspection of the pure culture plates.
Gram staining.
If Gram staining: Catalase, coagulase.
Inspection of the Sugar set, API-20E.
If Gram negative bacilli- do motility.
Inspection of the result of disc sensitivity.
REPORTING:
Wound swab M/E & culure report
Microscopy (Gram’s stain):
Film shows a few pus cells and a few epithelial cells. Organisms are Gram…+ve/-ve cocci/bacilli
Culture: Yielded the growth of ….at 370C in aerobic/anaerobic condition after 48 hours incubation.
Sensistivity: S or R.
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