 |
Reagent Preparation in Lab
|
01. 20 % Sulphosalicylic acid: For Urine protein
Sulphosalicylic acid ---------20 gm
Distilled water ---------------100 ml
02. 3% Glacial acetic acid: For Urine protein
Glacial acetic
acid----------- 03 ml
Distilled
water-------------- 100 ml
03.. Barium chloride (10% W/V): For Urine bile pigment
Barium chloride ------------
10 gm
Distilled
water---------------100 ml
04. Fouchets reagent: For urine Bile pigment
Trichloroacetic
acid--------- 25 gm
10% Ferric
chloride--------- 10 ml
Distilled water
------------- 100 ml
05. Ehrlich reagent: For Urine Urobilinogen
test
4-dimethylaminobenzaldehyde--
04 gm
Concentrated
HCl-------------- 40 gm
Distilled
water----------------- 160 ml
06. 3%
Sulphosalycylic acid: For Urine Myoglobin
Sulphosalycylic
acid-------03 gm
Distilled
water------------100 ml
07. Sodium nitroprusside reagent: For Urine
ketonebodies
Ammonium sulphate-----------------100gm
Sodium carbonates anhydrous ------50 gm
Sodium nitroprusside--------------3.0 gm
Note:
Grind
the crystals to a powder and use strong glass or plastic tube.
08. 20 % Potassium chromate: For Urine Chloride
Potassium
chromate-------20 gm
Distilled
water----------- 100 ml
09. 2.9 % Silver nitrate: For Urine Chloride
Silver nitrate
-------------- 29 gm
Distilled water------------ 1000 ml
10. Benzideine reagent: For Detection of blood
(Urine, stool)
Glacial acetic acid -------1.0 ml
Benzidine powder------ knife point
Hydrogen peroxide----- 1.0 ml
Note:
First
acid mixed with benzidine powder then equal volume H202
11. 3.5% Formal Saline: For Semen dilution
Sodium bicarbonate------- 05 gm
35% Formalin------------ 01 ml
Distilled water up to ---- 100 ml
12. Selivanoffs Reagent: For Semen Fructose
Resorcinol powder --------0.5 gm
HCL------------------------- 33 ml
Distilled water up to ---- 100 ml
13.
WBC Diluting fluid: For WBC’s count
Crystal violet ------------- 0.2 gm
Glacial acetic acid -------- 40 ml
Distilled water
---------- 200 ml
14.
CSF Diluting fluid: For CSF cell count
Crystal violet ------------ 0.2 gm
Glacial acetic acid ------- 10 ml
Distilled water
---------- 90 ml
15. Pandys reagent: For CSF Globulin
Phenol
crystal-------------10 gm
Distilled
water----------- 100 ml
16. 3% Trichloroacetic acid: For CSF Protein
Trichloroacetic
acid----- 03 ml
Distilled water----------
100 ml
17. 5% Potassium chromate: For CSF Chloride
Potassium
chromate-----05 gm
Distilled
Water---------- 100 ml
18. 0.5814% Silver nitrate: For CSF Chloride
Silver
ritrate---------- 5.814 gm
Distilled
water-------- 1000 ml
19. Carbol Fuchsine stain: For Z N stain
Basic fuchsine-----------10
gm
Ethanol or methanol
absolute--100 ml
Phenol-------------------50
gm
Distilled
water----------1000 ml
Here Basic Fushsin + Alconol and Phenol+ Distelled
water. Then mixed all .
20. 20 % Sulfuric acid: For Z N Stain
Sulfuric acid -----------20
ml
Distilled water-------- 100
ml
Note:
First
Distilled Water in container then sulfuric acid mixed slowly and cool water
flow out of the container as possible.
21. Methylene blue: For Z N Stain
Ethanol
----------------- 200 ml
M blue
------------------ 12 gm
Distilled
water ---------1000 ml
Here,
200 ml + 12 gm mixed, then add 1/Ltre (1000 ML) Distilled water.
22. Crystal Violet: For Gram stain
Crystal
violet--------------20 gm
Ammonium
oxalate------09 gm
Ethanol or
Methanol, absolute ----95 ml
Distilled
water----------- 1000 ml
23. Lugols iodine: For Grams stain
Potassium
iodide---------20 gm
Iodine---------------------10
gm
Distilled water
-------- 1000 ml
Here
First Potassium iodide D/water: Then iodine mixed.
24. Iodine Solu: For Faecal preparation
Potassium
iodide-------20 gm
Iodine--------------------10
gm
Distilled water
------ 1000 ml
Here,
First potassium iodide + Distilled water Then Idine mixed Keep it brown buttle.
25. Methyl red solution:
Methyl
red--------0.5 gm
Ethanol
absolute---28 ml
Distilled water
---- 22 ml
Here
Methyl red+ Ethanol add D/Water. Keep it dark place.
26. Neissers stain (Solution-A)
For corynebacterium diptherae
(KLB)
Methylene blue ---------01 gm
Absolute alcohol-------- 50 ml
Glacial acetic acid ------ 50 ml
Distilled water --------- 1000 ml
27. Neisser’s stain (Solution – B): For KLB
Crystal violet --------01 gm
Absolute alcohol --------10 ml
Distilled water --------- 300 ml
28. Neisser s solution: For Working
Solution A
-------02 Parts
Solution B
-------01 Part
Note: Mixed well and then use Filter with Filter
paper should be done before use.
29. Giemsa Stain: For Stain blood parasites
Giemsa Powder -------- 3.8 gm or
0.75 gm
Glyerine----------------- 250 ml or
35 ml
Methanol---------------- 250 ml or
65 ml
Mixed well and keep it brow bottle
Note. First Giemsa grind with glycerin and incubation
at 60 c for 02 hours.
Then
cool it and mixed Methanol
30. Crystal Violet: For capsule stain
Crystal violet----------01 gm
Distilled water -------100 ml
31. Acetone alcohol decolorize:
Acetone ----------------500 ml
Ethanol or methanol, absolute-----------475 ml
Distilled water------- --25 ml
32. Ammonium hydroxide, 30% v/v
Ammonia Solution conc.--15 ml
Distilled water--------------35 ml
33. Neutral red solution:
Neutral red--------------01 gm
Distilled water----------01 litre
34. Phenol saturated solution:
Phenol crystal---------- 02 gm
Distilled water--------- 30 ml
35. Tri sodium citrate (3.8% w/v): For ESR, PT
Tri sodium citrate --------3.8 gm
Distilled water----------- 100 ml
36. EDTA (Ethylene diamine tetra acetic acid):
For CP
Dipotassium ethylene diamine tetra acetate--20 gm
Distilled water---------------- 200 ml
37. Drabkins solution: Haemoglobin estimation.
Potassium ferricyanide ----------200 mg
Potassium cyanide -----------------50 mg
Potassium dihydrogen phosphate –140 mg
Nouidet P40 (Shell chemical co) – 01 ml
Distilled water ------------------1000 ml
Note: Keep it at room temperature in brown glass
bottle.
38. Crysodine solution: For Neister stain
Crysodine powder---1.0 gm
Distilled water ------100 ml
Note:
Dissolve by gently heating and filter and then use.
39. Leishman’s stain-
Leishman powder------0.15 gm
Acetone free methyl alcohol-- 100 ml
40. WBC Count fluid of blood-
Glacial acetic acid ---------1.5 ml
01% aqueous solution of Gention violet- 01 ml
Distilled water ------------------100 ml
41. RBC Count fluid of blood-
Sodium citrate --------------3.0 gm
Formalin---------------------- 01 ml
Distilled water --------------100 ml
42. Eosinophil fluid-
Eosin ------------------------ 0.1 gm
Acetone----------------------- 10 ml
Distilled water --------------100 ml
43. Platelet count fluid-
Ammonium oxalate---------01 gm
Distilled water --------------100 ml
44. 0.5 % Eosin solution : For Faccal preparation
Eosin powder--------------------0.5 gm
Distilled water -----------------100 ml
45. 60 Copper sulphate solution : For Specific gravity (Aspiration fluid)
Stock Solution:
Copper sulphate-----------------159 gm
Distilled water ------------------1000 ml
Working solution
|
Stock
Solution
|
Distilled
Water
|
Specific
Gravity
|
|
09ml
|
91ml
|
1010
|
|
10ml
|
90ml
|
1011
|
|
11ml
|
89ml
|
1012
|
|
12ml
|
88ml
|
1013
|
|
13ml
|
87ml
|
1014
|
|
14ml
|
86ml
|
1015
|
|
15ml
|
85ml
|
1016
|
|
16ml
|
84ml
|
1017
|
|
17ml
|
83ml
|
1018
|
|
18ml
|
82ml
|
1019
|
|
19ml
|
81ml
|
1020
|
|
20ml
|
80ml
|
1021
|
|
21ml
|
79ml
|
1022
|
|
22ml
|
78ml
|
1023
|
|
23ml
|
77ml
|
1024
|
|
24ml
|
76ml
|
1025
|
|
25ml
|
75ml
|
1026
|
|
26ml
|
74ml
|
1027
|
|
27ml
|
73ml
|
1028
|
|
28ml
|
72ml
|
1029
|
|
29ml
|
71ml
|
1030
|
FORMULA FOR
TOTAL PROTEIN
Sp
Gravity-1007 369 - 1000 = gm/dl
Ref Book :
Practical Clinical Biochemistry by Harold
Varely (4
th Ed ) Page no – 242
|
Sp
Gravity
|
Protein
(gm/dl)
|
|
1012
|
1.80
gm/dl
|
|
1013
|
2.20
gm/dl
|
|
1014
|
2.55
gm/dl
|
|
1015
|
2.90
gm/dl
|
|
1016
|
3.30
gm/dl
|
|
1017
|
3.65
gm/dl
|
|
1018
|
4.00
gm/dl
|
|
1019
|
4.40
gm/dl
|
|
1020
|
4.70
gm/dl
|
|
1021
|
5.20 gm/dl
|
|
1022
|
5.50
gm/dl
|
|
1023
|
5.80
gm/dl
|
|
1024
|
6.20
gm/dl
|
|
1025
|
6.60
gm/dl
|
|
1026
|
6.90
gm/dl
|
|
1027
|
7.30
gm/dl
|
|
1028
|
7.70
gm/dl
|
|
1029
|
8.00
gm/dl
|
|
1030
|
8.40
gm/dl
|
