Procedure of HbA1C Test

HbA1C Test

HbA1C:   A form of glycated haemoglobin. It means glucose chemically attaches to heamoglobin without help of enzyme.
Normal Hb
                  1. HbA1:  97 %
                  2. HbA2:  2.5 %
                  3. HbF  :   0.5 %
Normal distribution of HbA1:
1. HbA1a
2. HbA1b
3. HbA1c —-80%
Specimen:
1. EDTA blood / cp bottle
Patient Preparation:
1. No preparation required (not affected by food, exercise and time of collection.)
Method:
1. HPLC (High performance liquid chromatography )-  Biorad D-50
2. Capillary electrophoresis
What are the factors effecting HbA1C:
1. Blood glucose level
2. RBC life spam
Normal Range:-  4.2% – 6.4%
Indication:
Diabetic patient:
a. To asses blood glucose level of previous 8-10 weeks(2-2.5 months)
b. Diagnostic for diabetic
c. To asses risk of diabetic complacent.

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QUALITY CONTROL

                                       QUALITY CONTROL

Quality Control: Quality control may be defined as the study source of errors which are the responsibility of the clinical laboratory and the process used to recognized and minimize them.

  

Types of Quality Control: 

1. Internal quality control
2. External quality control 

Qus : Write down the terminology of quality control ?

Ans : 1.Accuracy : Ability of an analytical method to detect the test result, close to true value.
2. Precision: Ability of an analytical method to give the same result. If repeatedly in the same specimen for the same analyze.
3. Sensitivity: Ability of an analytical method to detect the smallest test quantity of substance than can be differentiated from zero.
4. Specificity: Ability of an analytical method to detect the substance that is specifically asked for.

Qus : Write down the purpose of quality control ?
Ans :
1. To asses the accuracy and precision of analytical method.
2. To asses the performance of laboratory equipments.
3. To asses the performance of laboratory persons.
4. To give the reliable test result.
5. To detect to minimize of errors.

Qus : Write down the type of errors?
Ans : 1.Basic error—Clerical error (Pre/Post analytical error)
2. Random error—Man made error (Analytical error)
3. Systemic error—Reagent /Machine error (Analytical error)

Qus : Write down the needs of quality control ?
Ans : 1.Man—-Laboratory person
2. Machine—-Laboratory equipment
3. Method—–Analytical method
4. Material—–Reagent, Control, Calibration.

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