Procedure of HbA1C Test

HbA1C Test

HbA1C:   A form of glycated haemoglobin. It means glucose chemically attaches to heamoglobin without help of enzyme.
Normal Hb
                  1. HbA1:  97 %
                  2. HbA2:  2.5 %
                  3. HbF  :   0.5 %
Normal distribution of HbA1:
1. HbA1a
2. HbA1b
3. HbA1c —-80%
Specimen:
1. EDTA blood / cp bottle
Patient Preparation:
1. No preparation required (not affected by food, exercise and time of collection.)
Method:
1. HPLC (High performance liquid chromatography )-  Biorad D-50
2. Capillary electrophoresis
What are the factors effecting HbA1C:
1. Blood glucose level
2. RBC life spam
Normal Range:-  4.2% – 6.4%
Indication:
Diabetic patient:
a. To asses blood glucose level of previous 8-10 weeks(2-2.5 months)
b. Diagnostic for diabetic
c. To asses risk of diabetic complacent.